CYSTEINE FUNCTIONALIZED BIO-NANOMATERIAL FOR THE AFFINITTY SENSING OF Pb(II) AS AN INDICATOR OF ENVIROMENTAL DAMAGE

MARÍA LAURA RAMÍREZ; CECILIA S. TETTAMANTI; FABIANA A. GUTIERREZ; JOSÉ M. GONZÁLEZ-DOMINGUEZ; ALEJANDRO ANSÓN-CASAOS; JAVIER HERNÁNDEZ-FERRER; MARÍA T. MARTÍNEZ; GUSTAVO A. RIVAS; MARCELA CECILIA RODRÍGUEZ

MICROCHEMICAL JOURNAL

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2018 vol. 141 p. 271 - 271

0026-265X

his work aims at the development of an electrochemical affinity biosensor forPb(II) quantification using a platform that combines glassy carbon electrodes(GCE) and an aqueous dispersion of single-walled carbon nanotubes (SWCNT)covalently modified with cysteine residues (Cys). The biosensing protocolincludes the accumulation of Pb(II) at the electrode surface through the affinityinteraction promoted by Cys residues at open circuit potential, followed by thereduction of the accumulated Pb(II) at -0.900 V and the transduction stepperformed by linear sweep-adsorptive stripping voltammetry (LSAdSV) in a0.020 M acetate buffer solution pH 5.00. There is a linear relationship betweenPb(II) oxidation peak current and Pb(II) concentration. The dynamic linear rangeextends from 5.0 to 125.0 gL-1, exhibiting a sensitivity of 0.061 Ag-1L and adetection limit of 0.69 gL-1. In addition, the selectivity of the biosensor wasevaluated in the presence of high concentration